Interferon alpha/beta R2 (IFN-alpha/beta Receptor 2, IFN-alpha Binding Protein, IFNAR2, IFN-R-2) (Carboxyfluorescein)

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£612.00

SKU:: I7661-59C
Application:: Flow Cytometry
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: 4°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Ser22-Ala239 (Lys160Asn) from human IFNa/b R2 expressed in NS0 cells.
Format:: Supplied as a liquid in saline containing up to 0.5% BSA, 0.09% sodium azide. Labeled with Carboxyfluorescein (CFS).
Extra Details:: IFN-a/b R2 belongs to the type II cytokine receptor family. It complexes with IFN-a/b R1 to form the signaling receptor complex for the family of a and IFN subtypes. By alternative splicing, IFN-a/b R2 can exist as a secreted soluble protein or as a type I membrane protein. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells using U937 human histiocytic lymphoma cell line. Optimal dilutions to be determined by the researcher. Flow Cytometry Validation This antibody has been tested for Flow Cytometry using A20 cells. 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul I7661-59C was added to 1-2.5 x 10e5 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in F4568-10 Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer. 4. The cells were resuspended in F4568-10 Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use. 5. Cell surface expression of IFN-a/b R2 was determined by flow cytometry using 488nm wavelength excitation and monitoring emitted fluorescence with a detector optimized to collect peak emissions at 515-545nm. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: Carboxyfluorescein conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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