I7661-45D
Integrin Beta 7 (Biotin)
Size
£991.00
- SKU:
- I7661-45D
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- CHO-cell derived recombinant human Integrin b7 aa22-723; isoform long extracellular domain.
- Format:
- Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.
- Extra Details:
- Human Integrin b7 (also Integrin bP and M290 IEL Ag) is a 120kD member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704aa extracellular domain (ECD) (aa20-723) and a 52aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa150-389) and four cysteine-rich EGF-like repeats. One splice variant exists that shows a deletion of aa501-648. Integrin b7 forms a nondisulfide linked heterodimer with a4 and aE integrins. a4b7 binds fibronectin, VCAM-1 and MADCAM1, while aEb7 binds E-cadherin. The ECD of human b7 is 87% aa identical to the ECD of mouse b7. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice