I7661-43F
Integrin, beta5 (Biotin)
Size
£965.00
- SKU:
- I7661-43F
- Application:
- WB
- Purity:
- Purified by human Integrin b5 affinity chromatography and then biotinylated.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- CHO cell-derived recombinant human Integrin b5, Gly24-Asn719.
- Format:
- Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
- Extra Details:
- Integrin β5 is a 100-110kD type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin αV. Integrin αVβ5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin β5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin β5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin β5, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Integrin β5. The detection limit for rhIntegrin β5 is approximately 2 ng/lane and 10 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 5% cross-reactivity with Integrin β1, -β2, -β3, rmIntegrin β4, -β6, and -β7. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice