I7661-39J-ML650
Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla) (MaxLight 650)
Size
£1,081.00
- SKU:
- I7661-39J-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Mouse Integrin, beta1. Species sequence homology: rat.
- Clone:
- 2Q841 (MB1.2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Integrin beta 1 is a 130kD transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Applications: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for use in Inhibition. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 2ug; lysate of 10e6 cell Immunohistochemistry (Frozen): As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed. Western Blot: on 10ug C2C12 lysate. C2C12 lysates was resolved by electrophoresis, transferred to PDVF membrane and probed with I7661-39J. Proteins were visualized using an IgG (HRP) goat anti-rat and a chemiluminescent detection system. Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins. Optimal dilution determined by the researcher. Positive Controls: Human OVCAR5 cell line whole cell lysate, C2C12 lysate. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice