I7661-39A3
Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Size
£479.00
- SKU:
- I7661-39A3
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human ocular melanoma cell line, V+B2
- Clone:
- 7H127
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Fixed paraffin sections Western Blot: 1-2ug/ml under non-reducing conditions Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of I7661-39A3 at a concentration of 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice