I7661-38

Integrin beta1, Activating

Size

£832.00

SKU:: I7661-38
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human IL-1b activated endothelial cells.
Clone:: 2Q849 (P4G11)
Format:: Supplied as a liquid in PBS, pH 7.1. No preservative added.
Extra Details:: Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, FACS and ELISA. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 2ug/ml (use on numerous cell lines as long as Ca2+ is present), Immunoprecipitation: 5ug/400ul lysate (0.5% triton PBS plus Ca++) FACS: 2-5ug/10e6 cells, in the presence of Ca++. Stimulation of cells: 2-10ug/ml per 10e6 cells. Binding buffer must contain 1mM Ca++ Immunohistochemistry: For use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and paraffin embedded (1:500, citrate HEIR treated, Qin et al 2003). Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.