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I7661-36D-FITC

Integrin alpha6 (VLA6, alpha6, beta1, alpha6 beta4, Laminin Receptors) (FITC)

Size

£1,081.00

SKU:
I7661-36D-FITC
Purity:
Purified by Protein A affinity chromatography
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Clone:
2Q956 (MA6)
Format:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:
Applications: Suitable for use in Immunoprecipitation, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Western Blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Immunocytochemistry: Cell lines tested by using antibody to express alpha 6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines. Optimal dilutions to be determined by the researcher. Cells lines that express Integrin alpha6: Mouse bone marrow-derived mast cells, melanoma cell line B16F1, ssquamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T-lymphocytes, thymocytes and various T-cell lines. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice