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I7661-36C

Integrin alpha6

Size

£825.00

SKU:
I7661-36C
Purity:
Purified
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6M guanidine isothiocyanate, and depleted of beta1 subunit
Clone:
2Q958 (BnC6B4 (a6C6))
Format:
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Extra Details:
Integrins are a family of heterodimeric membrane glycoproteins consisting on non-covalently associated alpha and beta subunits. In general, integrins function as receptors for extracellular matrix proteins. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation). Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor. Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation). Immunohistochemistry (Methanol-fixed cryosections): 35ug/ml An overnight methanol fix is suggested. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice