I7661-33C

Integrin alpha3, beta1 (CD49c, Very Late Antigen3a1b chain, VLA3a1b)

Size

£832.00

SKU:: I7661-33C
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human renal carcinoma cell line (KJ29).
Clone:: 2Q993 (M-KID2)
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: Integrins are a family of dimeric proteins that mediate cell-cell and extracellular matrix adhesion. Each VLA subtype (VLA-1, -2, -3, -4 and -5) is composed of a distinct alpha subunit noncovalently associated with a common beta subunit, as an alpha-beta dimer. Integrin VLA-3 (very late antigen, alpha 3/beta 1) is a cell surface receptor that binds to fibronectin, lamin, collagen type I and VI. VLA-3 is typically restricted in normal tissue but is widely expressed in many cultured tumor cells and correlates with the degree of invasiveness of malignant melanoma cells. Applications: Suitable for use in Immunohistochemistry, ELISA, RIA, Immunoprecipitation, Immunocytochemistry and FACS. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Acetone fixed, paraffin embedded sections Immunoprecipitation: Under non-reducing conditions, two chains with apparent molecular weight of 110 and 150kD. In the presence of a reducing agent (2% B-ME), a single molecular species of 130kD is detected. Under reducing conditions both alpha3 and beta1 subunits run with the same apparent molecular weight. Optimal dilutions to be determined by the researcher. Positive Controls: Cell Line: KJ29 renal carcinoma adherent cells Tissue: Kidney. Reacts with basal layer podocytes and basement membrane of distal tubules. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice