Integrin alpha3 (CD49c, Very Late Antigen3a chain, VLA3a) (Not for Export EU)

Size

£800.00

SKU:: I7661-33A
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal sequence, CRIQPSETERLTDDY, located in the cytoplasmic domain of the human alpha 3 integrin subunit.
Format:: Supplied as a liquid, 0.05% sodium azide.
Extra Details:: Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 19 alpha and 8 beta subunits that form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. Both of the subunits contribute to the binding of the ligand. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. Applications: Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: ELISA/RIA: 1:1000 Western Blot: 1:500 Detects Intergrin alpha 3 on 10ug of A431 lysates. Immunoprecipitation: 5ul precipitates alpha3/beta1 from 5x10e6 cells. Immunohistochemistry/Immunofluorescence: 1:500; Acetone-fixed tissue sections Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Positive Control: Human fetal foreskin fibroblasts (HFFF) neuroblastomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice