I7661-31J

Integrin, alpha1, beta1 (CD49a, VLA alpha1, Laminin Receptor, Collagen Receptor)

Size

£812.00

SKU:: I7661-31J
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CTL line directed against HLA-DR.
Clone:: 6A410 (TS2/7)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Human alpha 1 integrin sub-unit forms the VLA-1 heterodimer in association with the beta 1 integrin. VLA-1 is a receptor for collagen and laminin, and is expressed by chronically activated T cells, melanoma cells and smooth muscle cells. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:50. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Reagents: F(ab')2 rabbit anti mouse IgG:RPE I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.