I7661-27B

Insulin Receptor Substrate 1, Y612, Control Peptide (IRS-1)

Size

£512.00

SKU:: I7661-27B
Molecular Weight:: 1734
Purity:: 95+% HPLC, Mass Spec
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a white to off-white lyophilized powder.
Extra Details:: Insulin receptor substrate useful in phosphocellulose assay. Insulin Receptor Substrate-1 (IRS-1) is a major endogenous substrate for the insulin receptor and IGF-1R tyrosine kinase. Although IRS-1 is constitutively phosphorylated on serine and threonine, it is tyrosine-phosphorylated only after stimulation by insulin. The tyrosine phosphorylated IRS-1 serves as the major agent of insulin receptor-mediated signal transduction by being bound by cytoplasmic signaling proteins containing SH2 domains. Numerous SH2-containing signaling proteins (e.g., Grb2, SH-PTP2/SYP, PI-3-Kinase, and NCK) are known to preferentially bind to distinct tyrosine phosphorylation sites on IRS-1. Sequence (linear): H2N-Lys-Lys-His-Thr-Asp-Asp-Gly-Tyr-Met-Pro-Met-Ser-Pro-Gly-Val-Ala-OH Amino Acid Analysis: Confirms expected sequence Peptide Content: 80.0 ± 4% Solubility: H2O Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.