I7661-25G

Insulin Receptor, alpha (IRa)

Size

£646.00

SKU:: I7661-25G
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: IM-9 lymphocytes followed by purified insulin receptor. MW of Antigen: 135kD. Epitope: aa469-592 (exon 7/8). Cellular Localization: Cell membrane.
Clone:: 3F220
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration. The b-subunit (95kD) possesses a single transmembrane domain, whereas the a-subunit (135kD) is completely extracellular. Applications: Suitable for use in Immunoprecipitation, Tyrosine Kinase Assay and Inhibition. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Use Protein G): 2ug/mg protein lysate Tyrosine Kinase Assay: Antibody-mediated capture on microtiter plates Inhibits Insulin Binding: 10nM (1,2) Optimal dilutions to be determined by the researcher. Positive Control: IM-9 lymphocyte cells. Placenta. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.