I7661-21F
Insulin-Like Growth Factor I Receptor, alpha (IGF1Ra)
Size
£816.00
- SKU:
- I7661-21F
- Application:
- IP
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA. Epitope: aa184-283. Cellular Localization: Cell membrane.
- Clone:
- 3F218
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See I7661-21FX
- Extra Details:
- IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of a- and b-subunits, which are disulfide-linked in a b-a-a-b configuration in the mature receptor. The a-subunit is completely extracellular, while the b-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. Applications: Suitable for use in Immunoprecipitation, acts as a weak IGF-like Agonist in Bioassays and inhibits IGF-1 Binding. Not suitable for Western Blot. Other applications not tested. Recommended Dilutions: Bioassays: Thymidine incorporation into DNA Immunoprecipitation: 2ug/mg protein lysate. Use Protein A. IGF-1 Binding: 1.5ug/ml Substantial (>80%) inhibition Optimal dilutions to be determined by the researcher. Positive Control: Placenta or breast carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice