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I7661-19Q

Insulin-Like Growth Factor I Receptor, CT (IGF-I Receptor, IGFIR, IGFR, Insulin-like Growth Factor 1 Receptor, IGF1R, CD221, JTK13)

Size

£934.00

SKU:
I7661-19Q
Purity:
Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/1mg (LAL).
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Goat
Immunogen:
Recombinant protein corresponding to the extracellular domain of human Insulin-Like Growth Factor I Receptor.
Format:
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:
IGF­I receptor is a disulfide­linked heterotetrameric transmembrane protein consisting of two a and two b subunits. Both the a and b subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide­linked to yield the mature heterotetrameric receptor. The a subunit of IGF­I receptor is extracellular while the b subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF­I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF­I and II have been shown to be mediated via IGF­I R. Applications: Suitable for use in Western Blot, Immunohistochemistry, Neutralization and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Immunohistochemistry (paraffin): 5-15ug/ml using immersion fixed paraffin-embedded sections of human placenta (chorionic villi) and immersion fixed frozen sections of mouse embryo Neutralization: 0.5-1.5mg/ml in the presence of 6ng/ml recombinant human IGF-I. Immunocytochemistry:1-15ug/ml using immersion fixed MCF-7 human breast cancer cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice