I7661-17A1

Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) (Biotin)

Size

£479.00

SKU:: I7661-17A1
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IGFBP7, expressed in E coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.25-1.0ug/ml (100ul/well). The detection limit is 0.2-0.4ng/well in conjugation with I7661-17D as a capture antibody detects at least 0.2-0.4ng/well of recombinant hIGF-BP7. Western Blot: 0.1-0.2ug/ml detects human IGFBP7. Used in conjunction with compatible secondary reagents the detection limit for recombinant human IGFBP7 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.