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I7661-16

Insulin-Like Growth Factor Binding Protein 1 (IGFBP1) (Not for Export EU)

Size

£754.00

SKU:
I7661-16
Purity:
Serum
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
IGFBP-1 purified from human amniotic fluid.
Format:
Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS.
Extra Details:
IGFBP-1 (Insulin-like Growth Factor Binding Protein-1) is produced by the liver and regulated by glucocorticoids and insulin at the level of gene transcription. The N-terminal and C-terminal regions are highly homologous among rat, human and bovine sequences. IGFBP-1 inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 detects 10ng SDS-denatured human IGFBP-1. Immunoprecipitation: 1:500 precipitates >90% of 10ng IGFBP-1. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice