I7661-15E

Insulin-Like Growth Factor II (IGF2) (Biotin)

Size

£594.00

SKU:: I7661-15E
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Insulin Like Growth Factor II, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS. Labeled with Biotin. Reconstitute with PBS, 0.1% BSA to 0.1-1mg/ml.
Extra Details:: Insulin Like Growth Factor-II (IGF-II) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.25-1ug/ml using 100ul/well antibody solution, in conjunction with I7661-15D as a capture antibody, allows the detection of 0.2-0.4ng/well of recombinant hIGF2. Western Blot: 0.1-0.2ug/ml; The detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.