I7661-15D

Insulin-Like Growth Factor II (IGF2)

Size

£594.00

SKU:: I7661-15D
Purity:: Purified by affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human Insulin Like Growth Factor II, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Insulin Like Growth Factor II (IGF2) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml using 100ul/well antibody solution in conjunction with I7661-15E as a detection antibody, allows the detection of 0.2-0.4ng/well of recombinant hIGF-II. Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human IGF-II is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.