I7661-02B1

Insulin-Like Growth Factor I (IGF1, IGF I, IGF-I, IBP1, Mechano Growth Factor, MGF, Somatomedia C, Somatomedin C) (Biotin)

Size

£576.00

SKU:: I7661-02B1
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant human IGFI.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Extra Details:: IGF-I is a 70 amino acid secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver. IGF-I exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liver and lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumor cell growth. IGF-I signals through binding to the type 1 insulin-like growth factor receptor (IGF-1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. Production of IGF-I is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.25-1ug/ml. Can be used as a detection antibody in a sandwich ELISA with I7661-02B as the capture antibody. Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice