I7661-01F
Insulin-Like Growth Factor I (IGF1)
Size
£685.00
- SKU:
- I7661-01F
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to mouse IGF-1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
- Extra Details:
- IGF-1 stimulates the proliferation of a range of cell types including bone, cartilage tissue and muscle. Applications: Suitable for use in Neutralization, RIA, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml using 100ul/well will detect at least 2000-4000pg/ml of recombinant mouse IGF1 when used with a biotinylated antigen affinity purified anti-mouse IGF1 as the detection antibody at a concentration of approximately 0.2-0.4ug/ml. Western Blot: 0.1-0.2ug/ml when used with compatible secondary regeants. Neutralization: To yield one-half maximal inhibition-[ND50]-of the biological activity of mouse IGF-I (200ng/ml), a concentration of 12-14ug/ml of this antibody is required. Optimal dilutions to be determined by the researcher. Recommended Secondary Reagents: Donkey anti sheep/goat IgG: HRP conjugate Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice