Insulin

Size

£607.00

SKU:: I7660-25A
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the sequence of human insulin.
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: Glucose homeostasis is regulated by hormones. Elevations in blood glucose levels during feeding stimulates insulin release from pancreatic beta cells through a glucose sensing pathway. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to its secretion. A- and B-peptides are joined by a disulfide bond to form insulin, and the central part of the precursor molecule is cleaved and released as C-peptide. Insulin stimulates glucose uptake from blood into the skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:100 Immunofluorescence (Frozen): 1:100 Immunohistochemistry (Paraffin): 1:100. Antigen retrieval: heat in citrate buffer. Dilute antibody in TBST/5% normal goat serum. Flow Cytometry (Fixed/Permeabilized): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice