I7660-08

Insulin, Recombinant, Rat

Size

£369.00

SKU:: I7660-08
Molecular Weight:: 9.9
Purity:: ≥95% (SDS-PAGE, reducing). Endotoxin: ≤1.0EU per 1ug (LAL method).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a powder lyophilized from a solution containing PBS, pH 7.4, with 0.01% sarcosyl and 5% sucrose.
Extra Details:: Recombinant protein corresponding to aa25~54 and aa90-110 linked by a polypeptide linker (GGGGS), with two N-terminal Tags, His-tag and T7-tag expressed in E. coli. Swiss/Uniprot: P01323 Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSSIC SLYQLENYCN Predicted Molecular Weight: 9.9kD Subcellular Location: Secreted Predicted isoelectric point: 6.7 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and SDS-PAGE. ther Applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.