I7600-51-PE

Infectious Peritonitis, Feline (FIPV) (PE)

Size

£1,088.00

SKU:: I7600-51-PE
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Coronavirus cocktail
Clone:: 3H2460 (FIPV3-70)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Extra Details:: Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA, Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen, paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended. Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.