I7600-51-HRP
Infectious Peritonitis, Feline (FIPV) (HRP)
Size
£1,088.00
- SKU:
- I7600-51-HRP
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Coronavirus cocktail
- Clone:
- 3H2460 (FIPV3-70)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
- Extra Details:
- Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen, paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended. Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice