Immunoglobulin Superfamily Member 4C (IGSF4C, Nectin-Like 4, TSLC1-Like Protein 2, TSLL2)

Size

£774.00

SKU:: I7502-B70
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gln25-Tyr323 from the human IGSF4C extracellular domain, expressed in NS0 cells. Species Sequence Homology: Canine and mouse (99%)
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: IGSF4C (Immunoglobulin Superfamily member 4C), also known as Nectin-like 4 (Necl-4) and TSLL2, is a 48-55kD type I transmembrane glycoprotein that is a member of the nectin-like family of IGSF molecules. It is expressed in the brain, kidney, bladder and prostate. IGSF4C forms cis homophilic dimers on cell surfaces and mediates cell adhesion through homophilic trans-interactions in a Ca 2+/Mg 2+-independent manner. IGSF4C may also be a tumor-suppressor candidate in human prostate cancer. The mature 364 amino acid (aa) molecule contains a 299 aa extracellular domain (ECD) (aa 25-323) with three Ig-like motifs and a short cytoplasmic domain. There is at least one potential splice variant, which shows an insertion of 15 aa after Tyr309. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells using A172 human cell line Western Blot: 0.1ug/ml using recombinant Human IGSF4C/SynCAM4 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1903-12S, IgG, H&L (PE) Donkey x Goat I1903-30A, IgG, H&L, X-Adsorbed (FITC) Rabbit x Goat Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice