I6098-52BN

IgG2b, Mouse, Negative Control (FITC)

Size

£921.00

SKU:: I6098-52BN
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: 4°C
Supplier:: United States Biological
Host:: Mouse
Clone:: MPC-11
Format:: Supplied as a liquid. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: I6098-52BN is a control to approximate the amount of nonspecific binding of FITC conjugated mouse IgG2b monoclonal antibodies to human lymphocytes in a whole blood procedure with analysis by flow cytometry. It is suitable for use as negative isotype control for FC analysis using the primary antibody H6098-52A (anti-HLA Class 1 Antigen B27 (FITC) antibody). Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1ug (10ul) per 100ul whole blood. Optimal dilutions to be determined by the researcher. FITC/Protein Ratio: 6.0 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.