I3000-36A

IKK gamma (IKK3, NEMO, FIP3, IKKAP1, IkappaB Kinase gamma, IKKg)

Size

£594.00

SKU:: I3000-36A
Application:: WB
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: His-tagged full-length human IKK3/IKKg protein.
Clone:: 46B844
Format:: Supplied as a liquid in PBS, 0.05% sodium azide.
Extra Details:: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-7). IKK3/g preferentially interacts with IKK2/b and is required for activation of IKK complex. IKK3/g is also known as NEMO (NF-kB Essential MOdulator) (7). Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKa nor IKKb, but complexes directly with IKKg (8,9). This suggests that IKKg may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb (8). Applications: Suitable for detection of IKK3/IKKg expression by Western blot (10-12). Whole cell lysate from Jurkat or Daudi cells can be used as positive control and an approximately 48kD band is detected. Western blot analysis of 30ug of total cell lysate from Jurkat cells at 2ug/ml dilution. Recommended Dilution: Western blot: 2ug/ml Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice