I3000-11A1

Ikk alpha, beta, phosphorylated (Ser176) (IkappaB Kinase alpha, beta, IKKa,b)

Size

£359.00

SKU:: I3000-11A1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to human IKK-a/b around the phosphorylation site of serine 176 (Q-G-SP-L-C).
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins. Most agents that activate NF-kappaB do so through a common pathway based on phosphorylation- induced, proteasome-mediated degradation of IkappaB. The key regulatory step in this pathway involves activation of a high molecular weight IkappaB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKalpha and IKKbeta serve as the catalytic subunit of the kinase. IKKgamma serves as its regulatory subunit. Activation of IKK depends on phosphorylation, and the serines 177 and 181 in the activation loop of IKKbeta (176 and 180 in IKKalpha) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:4000 Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice