I3000-08J

IKK beta (IkappaB Kinase beta, IKKb, IKK2, IkBKb, IkappaB Kinase 2, Inhibitor of kappa Light Chain Gene Enhancer in B cells, Inhibitor of kappa Light Polypeptide Gene Enhancer in B cells Kinase beta, Inhibitor of Nuclear Factor kappa B Kinase beta, MGC131801, Nuclear Factor NFkB Inhibitor Kinase beta, NFKBIKB, Nuclear Factor of kappa Light Chain Gene Enhancer in B cells Inhibitor)

Size

£607.00

SKU:: I3000-08J
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full-length human IKKb/IKK2 recombinant protein .
Clone:: 8L373
Format:: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Extra Details:: NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 2-5ug per 1x10e6 cells Western Blot: 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Daudi, HeLa, Jurkat, or NIH 3T3 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice