I3000-04W

IKKB, phosphorylated (Tyr199) (Inhibitor Of Nuclear Factor kappa B Kinase beta Subunit, I-kappa-B-kinase beta, IkBKB, IKK-beta, IKK-B, I-kappa-B Kinase 2, IKK2, Nuclear Factor NF-kappa-B Inhibitor Kinase beta, NFKBIKB, IKBKB)

Size

£696.00

SKU:: I3000-04W
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr199 of human IKKb (KLH).
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the inhibitory I-kappa-B proteins by kinases (IKKa or IKKb) leads to the dissociation of the inhibitor/NF-kappa-B complex and marks the inhibitors for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.