I3000-01G

IkB alpha (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI) (Biotin)

Size

£679.00

SKU:: I3000-01G
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to aa32-391 from human IkB alpha. Species Sequence Homology: rat
Clone:: 6A920
Format:: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
Extra Details:: IkBa protein is a NF-kB inhibitory protein. It binds NF-kB, retains the complex in the cytoplasma, prevents it from entering nuclear and functioning as a transcription factor. Upon receiving a variety of signals, IkBa undergoes phosphorylation at serine residues by a ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds to DNA and affects gene expression. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.