I2500-09

IkB beta, CT (Not for Export EU)

Size

£914.00

SKU:: I2500-09
Purity:: Purified by delipidation and defibrination.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: IkB beta peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH. AA Sequence: RQP PSP ASK PLP DDP NPA
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Extra Details:: NFkB exists in the cytoplasm as a complex with a 38kDa inhibitor protein, IkB (MAD-3). Activation of NFkB by different stimuli, including TNFa and PMA, requires the dissociation of the inhibitor protein. Dissociation is regulated by the phosphorylation of IkB on serine residues 32 and 36 which targets IkB for ubiquinitation and proteasome mediated degradation. The NFkB complex then translocates to the nucleus and activates transcription of responsive genes. Application(s): Suitable for Immunoprecipitation, Western Blot, ELISA. Recommended Dilution: ELISA: 1:1000-1:5000 Western Blot: 1:500-1000 (Peroxidase conjugated goat anti-rabbit IgG (H&L). Anti-IkB beta is suitable for the detection by immunoblot of human, mouse and rat IkB beta. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.