I2500-05A
IkB alpha (IkBa)
Size
£512.00
- SKU:
- I2500-05A
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- E. coli-expressed mouse IkBa
- Format:
- Supplied as a lyophilized powder in 0.09% sodium azide.
- Extra Details:
- IkBa protein is a NF-kB inhibitory protein. It binds NF-kB, retains the complex in the cytoplasma, prevents it from entering nuclear and functioning as a transcription factor. Upon receiving a variety of signals, IkBa undergoes phosphorylation at serine residues by a ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds to DNA and affects gene expression. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice