I2500-01
IkB alpha
Size
£576.00
- SKU:
- I2500-01
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the sequence surrounding Arg29 of human IκBα.
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB proteins (1-3). Activation occurs via phosphorylation of IkappaB-alpha at Ser32 and Ser36, resulting in the release and nuclear translocation of active NF-kappaB (3-7). IkappaB-alpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals, including inflammatory cytokines, growth factors and chemokines. Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin followed by proteasome-mediated degradation of IkappaB, resulting in the release of active NF-kappaB. Kinases that phosphorylate IkappaB at these activating sites have been identified (8). Because phosphorylation of IkappaB-alpha at Ser32 is essential for release of active NF-kappaB, phosphorylation at this site is an excellent marker of NF-kappaB activation (1-3). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice