IgM (mu chain specific) (FITC)

Size

£608.00

SKU:: I1905-15M1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Chicken IgM u (mu chain)
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Fluorescein isothiocyanate (FITC) (MW 390 daltons) Reconstitution: 1.0ml sterile dH2O.
Extra Details:: Applications: Suitable for use in FLISA, Immunofluorescence Microscopy and Flow Cytometry. Other applications not tested. Recommended Dilution: FLISA: 1:10,000 - 1:50,000 IF Microscopy: 1:1,000 - 1: 5,000 Flow Cytometry: 1: 500 - 1:2,500 Optimal dilutions to be determined by the researcher. Absorption Wavelength: 495nm Emission Wavelength: 528nm Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Goat IgG Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.