IgM (mu Chain), X-Adsorbed (HRP)

Size

£345.00

SKU:: I1905-04C6
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified Human IgM, µ chain
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% proclin 150, 1% BSA (Protease/IgG free), 0.1% Kathon CG. Reconstitute with 1.1 ml ddH2O and let stand 30 minutes at room temperature to dissolve. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000 Western Blot: 1:10,000-1:50,000 Immunohistochemistry: 1:500-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.