I1905-03F-FITC

IgM, Fc (FITC)

Size

£895.00

SKU:: I1905-03F-FITC
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: IgM from human serum
Clone:: 5E669
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Applications: Suitable for use in FLISA, Western Blot, and detection of IgM antibodies and total IgM. Can also be used as labeled reagent for IgM or mu-chains detection by means of Western Blot and for passive hemagglutination. Other applications have not been tested. Recommended Dilution: Indirect FLISA: 5x10e-7 Optimal dilutions to be determined by the researcher. Hybridoma: Derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with IgM from human serum. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.