I1904-89D

IgG4 (HRP)

Size

£1,231.00

SKU:: I1904-89D
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified IgG4.
Clone:: 3H2426 (HP6025)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal. Labeled with Horseradish Peroxidase (HRP).
Extra Details:: Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: 1:1000-1:20,000 Immunohistochemistry: Paraffin sections Immunohistochemistry (Frozen): 1:40-1:80. Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Tonsil Hybrdoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.