IgG3 (Biotin)

Size

£623.00

SKU:: I1904-85G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Mouse IgG3 heavy chain.
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Extra Details:: Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested. Recommended Dilution: ELISA: 1:8000 to 1:32,000 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:500-1:2000 Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.