I1904-84H-HRP

IgG2b (HRP)

Size

£895.00

SKU:: I1904-84H-HRP
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat IgG2b myeloma protein (IR863).
Clone:: MARG 2b-8
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA: 5ug/ml (as a coating antibody) Optimal working dilutions to be determined by researcher. Avidity for rat IgG2b: 4.7 x 10e9 M-1. Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.