I1904-83N
IgG2a (Biotin)
Size
£623.00
- SKU:
- I1904-83N
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Mouse IgG2a heavy chain
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
- Extra Details:
- Applications: Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Other applications not tested. Recommended Dilution: ELISA: 1:30,000-1:150,000; I1904-83N was assayed against 1ug of mouse IgG2a in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilutions to be determined by the researcher. Reconstitution: Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice