I1904-80I-HRP

IgG2a (HRP)

Size

£1,151.00

SKU:: I1904-80I-HRP
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Purified IgG2a from BALB/c mice.
Clone:: 3H2348 (LO-MG2a-9)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 5ug/ml as coating antibody Optimal dilutions to be determined by the researcher. Avidity: 2.5x10e9Me-1 Recommeded Pair: Coating: I1904-80I Detection: I1904-83G1 Hybridoma: Spleen cells from immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.