I1904-65M
IgG (Biotin)
Size
£623.00
- SKU:
- I1904-65M
- Purity:
- Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Mouse IgG g heavy chain
- Format:
- Biotin conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
- Extra Details:
- Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule Application(s): Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilution(s): This product has been assayed against 1.0 m g of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Storage Conditions: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with 50% glycerol and then aliquot contents and freeze at-20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- Shipping Conditions:
- Blue Ice