IgG, F(ab')2 (AP)

Size

£656.00

SKU:: I1904-59X
Purity:: Purified by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Sheep IgG F(ab’)2 fragment
Format:: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.
Extra Details:: Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140kD) Applications: Suitable for Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500 to 1:2,000; using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.