IgG, Fc, X-Adsorbed (HRP)

Size

£634.00

SKU:: I1904-59R
Purity:: Prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Sheep IgG F(c) fragment
Format:: Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Thimerosal (addition of sodium azide not recommended). Labeled with horseradish peroxidase (HRP).
Extra Details:: Label: Horseradish Peroxidase (HRP) Application(s): Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry. Recommended Dilution(s): Immunohistochemistry: 1:500 to 1:5000 on tissue sections ELISA: 1:5000-1:50,000 Western Blot: 1:5000-1:50,000 Optimal titers for other applications should be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.