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I1904-54

IgG, F(ab’)2 (Biotin)

Size

£576.00

SKU:
I1904-54
Purity:
≥ 99% Purified by Rat IgG-agarose affinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, rat IgG and rat serum. Biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Rat IgG F(ab’)2 fragment.
Format:
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, 0.01% thimerosal.
Extra Details:
Applications: Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy and antibody based assays using streptavidin or avidin conjugates. Recommended Dilution: Capture ELISA: 1:6000-1:25,000 of the reconstitution concentration is suggested Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice