IgG H&L, X-adsorbed (HRP)

Size

£659.00

SKU:: I1904-50V1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Rat IgG, whole molecule. Label: Horseradish Peroxidase (HRP) (MW: 40,000 daltons)
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin Sulfate. Reconstitute with 1ml sterile dH2O.
Extra Details:: Applications: Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy. Other applications not tested. Recommended Dilutions: ELISA: 1:3000 to 1:12,000 using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.