IgG, F(ab’)2 (HRP)

Size

£577.00

SKU:: I1904-49H
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Rat IgG F(ab’)2 fragment.
Format:: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for use in Western Blot, Dot Blot, ELISA, Immunopreoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:500-1:3000. 1ug of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis- [3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.