IgG, Fc (HRP)

Size

£320.00

SKU:: I1904-49C
Purity:: Prepared by immunoaffinity chromatography followed by solid phase adsorption.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Rat IgG F(c) fragment
Format:: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Extra Details:: Applications: Suitable for Western or Dot Blot, ELISA, Immunoperoxidase, Electron Microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:50,000. Assayed against 1ug of Rat IgG in a standard capture ELISA. Immunohistochemistry:1:500-1:2500 Western Blot: 1:1000-1:10,000 Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.